Immunohistochemical analysis of NPNT in immersion fixed paraffin-embedded sections of mouse pancreas. Samples were incubated with NPNT polyclonal antibody (Product # PA5-47610) using a dilution of 1 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells.